Anti-DPGA anthrax [11D ]

Absolute Antibody

Anti-DPGA anthrax [11D ]

9

This antibody specifically binds the poly-gamma-D-glutamic acid (gammaDPGA) capsule of Bacillus anthracis. Bacillus anthracis is surrounded by an antiphagocytic polypeptide capsule composed of poly gamma-d-glutamic acid (gammaDPGA). The gammaDPGA capsule

The binding characterization of this antibody to gammaDPGA of Bacillus anthracis was done using ELISA. The binding affinity of the antibody was measured using surface plasmon resonance. It was reported that the IgG1 version of this antibody binds 10-mer peptide of gammaDPGA with a binding affinity of Kd= 0.3 nM. It was reported that a single 30-microg dose of this antibody when given to BALB/c mice 18 h before challenge conferred about 50% protection against a lethal intratracheal spore challenge by the virulent B. anthracis Ames strain. When given 8 h or 20 h after challenge, this antibody provided significant protection against lethal infection (PMID: 21187383). This antibody can be used in treatment of infections with antibiotic-resistant strains (PMID: 22069754).

ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

11D

Mouse

IgM

Bacteria

-20°C,2°C to 8°C

12352203