Anti-DPGA anthrax [F26G3]

Absolute Antibody

Anti-DPGA anthrax [F26G3]

9

This antibody specifically binds the poly-gamma-D-glutamic acid (gammaDPGA) capsule of Bacillus anthracis. Bacillus anthracis is surrounded by an antiphagocytic polypeptide capsule composed of poly gamma-d-glutamic acid (gammaDPGA). The gammaDPGA capsule

The binding of this antibody to B. anthracis capsule was assessed by direct immunofluorescence using the Alexa Fluor 488 (Molecular Probes) labeled antibody. An antigen capture ELISA was developed, where this antibody was used as capture antibody and a horseradish peroxidase conjugate of this antibody was used as an indicator antibody. The assessment of assay sensitivity was done using gammaDPGA from B. licheniformis demonstrated that the assay could detect gammaDPGA at concentrations as low as 100-140 pg/ml. Passive immunization with this antibody protected >90% of mice in a pulmonary model of anthrax that was lethal in control mice (PMID: 15051894). A 50:50 mixture of PDGA antibodies F24F2 and F26G3 was used in the development of an antigen capture ELISA with a sensitivity of approximately 9 ng/ml of serum after a 1/40 dilution and 2.25 ng/ml for a 1:10 dilution (PMID: 19506008).

ImmunoFluorescence, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

F26G3

Rabbit

IgG

Bacteria

-20°C,2°C to 8°C

12352203