Anti-E Cadherin [19A11]

Absolute Antibody

Anti-E Cadherin [19A11]

9

This antibody recognizes the N-terminal extracellular cadherin repeat domain 1 (EC1) of the human E-cadherin. This antibody also cross reacts with canine E-cadherin. Cadherins are calcium-dependent cell adhesion proteins. It is localized on the surfaces o

The initial screeding of E-Cadherin-positive hybridoma supernatants for binding to live Colo 205 cells was done using an ELISA. Activating antibody 19A11 stimulated the adhesion of Colo 205 cells to pure E-cadherin substrate (PMID: 22513089). This antibody is also capable of recognizing denature human E-cadherin in a western blot. This antibody also inhibited the formation of epithelial tubules, causing the cells to remain as round epithelial cysts. This antibody also enhanced the adhesion of HGF-treated MDCK cells to E-cadherin when analyzed with the flow adhesion assay (PMID: 22513089). This antibody slowed the rate of wound closure in both normal culture medium and HGF-treated cultures (PMID: 22513089). This antibody is an E-cadherin activating antibody which stimulates the apoptosis of circulating tumor cells (PMID: 32127478). This antibody was used in determination of expression of E-cadherin on low-adherent Colo205 cells using flow cytometry (PMID: 27582386).

Flow Cytometry, Western Blot, ELISA, Other Application

Research Use Only

Monoclonal

19A11

CDH1

cadherin 1

Arc-1; BCDS1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDHE; cell-CAM 120/80; ECAD; E-cadherin 1; epididymis secretory sperm binding protein; epithelial cadherin; LCAM; UVO; uvomorulin

Human

IgG1

Cadherin-1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Canine, Human

-20°C,2°C to 8°C

12352203