IF analysis of COL4A1/E Cadherin using anti-COL4A1/E Cadherin antibody (PB9099/M00063-2) COL4A1/E Cadherin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-COL4A1 Antibody (PB9099)/mouse anti E Cadherin Antibody(M00063-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) /Cy3 conjugated Goat anti mouse IgG(BA1031),were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of COL4A1/E Cadherin using anti-COL4A1/E Cadherin antibody (PB9099/M00063-2) COL4A1/E Cadherin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-COL4A1 Antibody (PB9099)/mouse anti E Cadherin Antibody(M00063-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) /Cy3 conjugated Goat anti mouse IgG(BA1031),were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Anti-E Cadherin 1 CDH1 Antibody Picoband(r) (monoclonal, 9G2)
M00063-2
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCDH1
Overview
- SupplierBoster Bio
- Product NameAnti-E Cadherin Antibody (monoclonal, 9G2)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9G2
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID999
- Target nameCDH1
- Target descriptioncadherin 1
- Target synonymsArc-1; BCDS1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDHE; cell-CAM 120/80; ECAD; E-cadherin 1; epididymis secretory sperm binding protein; epithelial cadherin; LCAM; UVO; uvomorulin
- HostMouse
- IsotypeIgG1
- Protein IDP12830
- Protein NameCadherin-1
- Scientific DescriptionBoster Bio Anti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) catalog # M00063-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7. Deng B et al., 2015 Sep, Tumour BiolRead more