Anti-E protein [14D5]

Absolute Antibody

Anti-E protein [14D5]

7

The specificity of the IgG1 format was confirmed by ELISA analysis. The antibody was able to neutralize infectivity of TBEV in vitro (Tsekhanovskaya et al. 1993; PMID: 7505512). Two chimeric versions of the antibody were constructed, ch14D5a and ch14D5b, the affinity constants of which were 2.6 x 10^10 M-1 and 1.0 x 10^7 M-1, respectively, as determined by surface plasmon resonance. The chimeric ch14D5a and parental antibodies were analyzed for the neutralizing activity against TBEV strain 205 by FRNT. The neutralization index (IC50) of the chimeric version was 0.04 microg/ml and 0.5 microg/ml for the parental version. In vivo experiments using the chimeric ch14D5a version resulted in a 100% survival of the mice infected with 240 LD50 of TBEV at a dose of 10 microg/mouse. This chimeric antibody is promising for further development of prevention and therapeutic drugs against TBEV (Baykov et al., 2014; PMID: 24837772). The antibody was employed for in vivo experiments in BALB/c mice infected with TBEV, at doses of 100 microg and 10 microg of antibody per mouse. The antibody showed protective efficacy when injected at the high dose one day after infection, with survival rates that were TBEV dose-dependent, and complete protection when it was injected one day before infection. No protection was observed in mice received the low dose of the antibody (Matveev et al., 2020; PMID: 32409136). The crystal structure of the Fab fragment of the chimeric antibody in complex with the D3 domain of TBEV glycoprotein E was determined (Baykov et al., 2021). The antibody detected the EIII protein by western blot analysis (Stenkova et al., 2017; PMID: 28741465). Immunofluorescence was performed on Vero E6 cells using this antibody (Levanov et al. 2014, PMID: 24371235).

ELISA, Neutralisation/Blocking, Other Application

ELISA; in vitro; in vivo; Neutralization; SPR; Crystallization

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203