Anti-E1 protein [DC1.7]

Absolute Antibody

Anti-E1 protein [DC1.7]

9

This antibody binds and epitope near the E1 DIII domain of the Chikungunya virus (CHIKV), which is the causative agent of zoonotic disease in humans called Chikungunya. Additionally, this antibody also cross reacts with multiple arthritogenic alphaviruses

The binding characterization of the IgG antibody for CHIKV E1 protein was done using ELISA and Biolayer interferometry (BLI). This antibody was capable of binding biotinylated p62-E1 hybrid protein in a flow cytometric assay. This antibody was also capable of immunoprecipitating the E1 envelope protein of CHIKV. This antibody was can neutralize CHIKV 181/25 and CHIKV LR2006_OPY1 with IC50 value in the low nanomolar range and 13nM respectively. This antibody could not provide sufficient in vivo protection from CHIKV infection (PMID: 31697791).

Flow Cytometry, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

DC1.7

CHIKVgp2

structural polyprotein

CHIKVgp2; structural polyprotein

Human

IgG1

Structural polyprotein

Virus

-20°C,2°C to 8°C

12352203