Anti-Ebola surface glycoprotein [KZ52]

Absolute Antibody

Anti-Ebola surface glycoprotein [KZ52]

7

This antibody neutralizes Zaire ebolavirus in vitro and offers protection from lethal EBOV challenge in rodent models, but has minimal effects on viral pathogenicity in non-human primates. KZ52 likely neutralizes by preventing rearrangement of the GP2 HR1A/HR1B segments and blocking host membrane insertion of the internal fusion loop. Alternatively, IgG KZ52 may sterically hinder access to the RBS or to a separate binding site of another cellular factor, especially if multiple attachment events are required for entry (Lee et al. 2009). This antibody will detect both live and fixed virus by IF (Maruyama 1999, PMID: 10364354) . It has also been used as a positive control in the development of anti-Ebola polyclonal goat antibodies (Boisen et al. 2015).

ImmunoFluorescence, ImmunoPrecipitation, Neutralisation/Blocking, Other Application

NTRL; IF; IP; crystallization

Research Use Only

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203