Anti-EDIII of DENV2 [3H5]

Absolute Antibody

Anti-EDIII of DENV2 [3H5]

9

The antibody binds to domain III of the envelope (E) protein of Dengue virus. In particular, it targets residues buried between E dimers and located close to the viral membrane. The antibody is serotype specific to DENV2. The E protein is a structural pro

Binding avidity/affinity of both Full length (IgG) and Fab fragments of the antibody to whole virion measured by ELISA. The crystal structure of the Fab fragment in complex with EDIII of DENV2 NGC was determined. Focus reduction neutralization assays showed highly potent neutralization of DENV2 NGC and 16681 for the IgG1 fragment with 50% neutralization titers in the sub-nanomolar range (50% FRNT at 0.044 nM ). Pre- and postattachment neutralization assays showed the antibody is capable of significantly inhibiting infection after the virus had bound to the cells. The antibody promotes little antibody dependent enhancement (ADE). ADE was analyzed using U937 cells, the antibody showed no enhancing capacity for DENV2 NGC and reduced enhancement of DENV2 16681 at a very narrow concentration range. Also the IgG2a format of the antibody showed no enhancement of infection for DENV2 NGC while still potently neutralizing infection. The humanized version of the antibody (human IgG1) showed little enhancement for DENV2/16681 and DENV2/NGC. Surface plasmon resonance assay was performed to evaluate the binding specificity and affinity between the antibody and recombinant EDIII (Kd= 1,0 nM). The antibody showed potential as therapeutic (Renner et al., 2018; PMID: 30323338). Antibody-dependent infection enhancement (ADE) was reported for the antibody using P-388D1 mouse macrophage-like cells (Morens et al., 1987; PMID: 3504546). This antibody was used for flow cytometry on wild-type DIII and mutant DIII expressed on the yeast surface (Sukupolvi-Petty et al., 2007; PMID: 17881453). The antibody was reported to cause ADE in THP-1 cells at low doses however, it did not enhance DENV-2 infection of AG129 mice. Fifty-percent plaque reduction neutralization test (PRNT50) values of the antibody determined by using 100 PFU of S221 (PRNT50 values of 0.19 ug) (Watanabe et al., 2015; PMID: 25787279). The antibody was labeled with Eu* conjugate and used in a sandwich ELISA for the detection of DENV-2 (Shukla et al., 2018; PMID: 29367852)

Flow Cytometry, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

3H5

Mouse

IgM

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Virus

-20°C,2°C to 8°C

12352203