Anti-EGFR [2224]

Absolute Antibody

Anti-EGFR [2224]

9

This antibody is specific for human EGFR.

This antibody is derived after affinity maturation of a parental anti-EGFR clone C10. The original scFv antibody showed a 280 fold increase in affinity compared to the parental scFv clone C10. This antibody was used for analysis of scFv-mediated internalization of fluorescent immunoliposomes and quantification by microscopy and flow cytometry. This antibody bound to human EGFR expressed on MDAMB468 cells, A431 cells and EGFR (vIII) stably transfected NR6M and was detected by flow cytometry (Zhou et al, 2007; pmid:17602702).

Flow Cytometry

Research Use Only

Monoclonal

2224

EGFR

epidermal growth factor receptor

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1

Mouse

IgG2b

Epidermal growth factor receptor

This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203