
Figure 1. Western blot analysis of EIF3E using anti-EIF3E antibody (A00481-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3E antigen affinity purified polyclonal antibody (Catalog # A00481-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF3E at approximately 52 kDa. The expected band size for EIF3E is at 52 kDa.
Anti-EIF3E Antibody Picoband(r)
A00481-4-FITC
TargetEIF3E
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-EIF3E Antibody Picoband(r)
- Delivery Days Customer9
- Concentration500 ug/ml
- Gene ID3646
- Target nameEIF3E
- Target descriptioneukaryotic translation initiation factor 3 subunit E
- Target synonymseIF-3 p48; eIF3-p46; EIF3-P48; EIF3S6; eukaryotic translation initiation factor 3 subunit 6; eukaryotic translation initiation factor 3 subunit E; eukaryotic translation initiation factor 3, subunit 6 (48kD); eukaryotic translation initiation factor 3, subunit 6 48kDa; INT6; mammary tumor-associated protein INT6; murine mammary tumor integration site 6 (oncogene homolog); viral integration site protein INT-6 homolog
- Protein IDP60228
- Protein NameEukaryotic translation initiation factor 3 subunit E
- Scientific DescriptionBoster Bio Anti-EIF3E Antibody Picoband® catalog # A00481-4. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203