Figure 1. Western blot analysis of Elovl4 using anti-Elovl4 antibody (A03840-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Elovl4 antigen affinity purified polyclonal antibody (Catalog # A03840-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Elovl4 at approximately 37 kDa. The expected band size for Elovl4 is at 37 kDa.
Figure 1. Western blot analysis of Elovl4 using anti-Elovl4 antibody (A03840-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Elovl4 antigen affinity purified polyclonal antibody (Catalog # A03840-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Elovl4 at approximately 37 kDa. The expected band size for Elovl4 is at 37 kDa.
Anti-Elovl4 Antibody Picoband(r)
A03840-1-DYLIGHT488
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetElovl4
Overview
- SupplierBoster Bio
- Product NameAnti-Elovl4 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 488
- Gene ID83603
- Target nameElovl4
- Target descriptionelongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
- Target synonyms3-keto acyl-CoA synthase Elovl4; elongation of very long chain fatty acids protein 4; ELOVL FA elongase 4; ELOVL fatty acid elongase 4; very long chain 3-ketoacyl-CoA synthase 4; very long chain 3-oxoacyl-CoA synthase 4
- HostRabbit
- IsotypeIgG
- Protein IDQ9EQC4
- Protein NameElongation of very long chain fatty acids protein 4
- Scientific DescriptionBoster Bio Anti-Elovl4 Antibody Picoband® catalog # A03840-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203