Bio-Connect

Anti-Endomucin [v5c7.8]

AB02441-3.0-BT
Absolute Antibody
ApplicationsFunctional Assay, Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetEmcn
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Endomucin [v5c7.8]
  • Delivery Days Customer
    9
  • Antibody Specificity
    This antibody is specific for carbohydrate epitopes of endomucin. The epitopes were resistant to endoglycosidase F. Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complex
  • Application Supplier Note
    In an attempt to identify the antigen to which this antibody binds multiple experiments were preformed. First, immunohistochemistry was preformed on normal mouse organs using the rat version of this antibody. Further, flow cytometry was preformed on mouse cells using the rat version of this antibody. This clone was also used successfully in immunoprecipitation and the immunoprecipitated proteins were immunoblotted using the mouse version of this antibody (Morgan et al, 1999; pmid:9864158). To analyse the expression of endomucin during development of the vascular system immunohistochemistry was preformed on tissue from mouse embryos using the mouse version of this antibody (Brachtendorf et al, 2001; pmid:11747076). While characterizing antibodies against human endomucin using immunohistochemistry, the rat version of this antibody was used as a negative control (Samulowitz et al, 2002; pmid:12000719). To better understand the influence of blood flow on hematopoietic stem and progenitor cell (HSPC) homing to the bone marrow (BM) microvasculature of the calvarial bone two-photon intravital microscopy was preformed on a moiuse using the rat version of this antibody (Stewen and Bixel, 2019; pmid:31197772). The mouse version of this antibody was also used for immunohistochemistry on human pancreatic cancer cell lines to asssess antitumor activity of nintedanib (Awasthi et al, 2015; pmid:25527450). To eludicate the mechanisms behind the ability of antiangiogenic therapy to stimulate cancer cell invasion and metastasis immunohistochemistry was preformed on tumor cells from KIC transgenic mice (Aguilera et al, 2013; pmid:24346431).
  • Applications
    Functional Assay, Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    v5c7.8
  • Gene ID59308
  • Target name
    Emcn
  • Target description
    endomucin
  • Target synonyms
    0610012K22Rik; AI315669; endomucin; endomucin-1; endomucin-1/2; Muc14; MUC-14; mucin 14, endothelial; mucin-14
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ9R0H2
  • Protein Name
    Endomucin
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203