anti-eNOS phospho (Ser1177) antibody
ARG20524
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNOS3
Overview
- SupplierArigo Biolaboratories
- Product Nameanti-eNOS phospho (Ser1177) antibody
- Delivery Days Customer23
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID4846
- Target nameNOS3
- Target descriptionnitric oxide synthase 3
- Target synonymscNOS; constitutive NOS; ECNOS; EC-NOS; endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase, endothelial; NOS type III; NOSIII
- HostRabbit
- IsotypeIgG
- Scientific DescriptionNitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes, inducible NOS (iNOS, NOS-II), neuronal NOS (bNOS, NOS-I), and endothelial NOS (eNOS, ecNOS, NOS-III). Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation. Tyr-657 is phosphorylated by PYK2 in response to fluid shear stress and this phosphorylation leads to attenuation of enzyme activity.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203