Anti-Envelope protein domain III [POWV-80]

Absolute Antibody

Anti-Envelope protein domain III [POWV-80]

7

The specificity of the antibody (IgG2c) was determined by ELISA. The antibody was used for flow cytometric analysis of HEK-293T cells. The antibody showed neutralizing activity on Vero cell incubated with POWV MA51240. The antibody showed neutralizing activity also against TBEV and LGTV (EC50 respectively 8.4 and 103 ng/ml). The binding affinity of the antibody to POWV E was measured using biolayer interferometry (BLI), showing the antibody binds POWV E with an affinity (KD) of ~30 nM. The crystal structure of the Fab fragment in complex with POWV DIII was determined. The antibody might have therapeutic potential (VanBlargan et al., 2021; PMID: 33831142).

Flow Cytometry, ELISA, Neutralisation/Blocking, Other Application

ELISA; Neutralizing; FC; Crystallography

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2c format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203