Figure 1. IHC analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066). Eotaxin 3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Eotaxin 3 Antibody (PB9066) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066). Eotaxin 3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Eotaxin 3 Antibody (PB9066) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Eotaxin 3/CCL26 Antibody Picoband(r)
PB9066-10UG
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCCL26
Overview
- SupplierBoster Bio
- Product NameAnti-Eotaxin 3/CCL26 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for Eotaxin 3 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID10344
- Target nameCCL26
- Target descriptionC-C motif chemokine ligand 26
- Target synonymsCC chemokine IMAC; C-C motif chemokine 26; chemokine (C-C motif) ligand 26; chemokine N1; eotaxin-3; IMAC; macrophage inflammatory protein 4-alpha; MIP-4a; MIP-4alpha; MIP-4-alpha; SCYA26; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; thymic stroma chemokine-1; TSC-1
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y258
- Protein NameC-C motif chemokine 26
- Scientific DescriptionBoster Bio Anti-Eotaxin 3/CCL26 Antibody Picoband® catalog # PB9066. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203