
Figure 1. Western blot analysis of Epac1/RAPGEF3 using anti-Epac1/RAPGEF3 antibody (A02483-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: monkey COS7 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epac1/RAPGEF3 antigen affinity purified polyclonal antibody (Catalog # A02483-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epac1/RAPGEF3 at approximately 104 kDa. The expected band size for Epac1/RAPGEF3 is at 104 kDa.
Anti-Epac1/RAPGEF3 Antibody Picoband(r)
A02483-3-DYLIGHT594
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetRAPGEF3
Overview
- SupplierBoster Bio
- Product NameAnti-Epac1/RAPGEF3 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID10411
- Target nameRAPGEF3
- Target descriptionRap guanine nucleotide exchange factor 3
- Target synonyms9330170P05Rik; bcm910; CAMP-GEFI; cAMP-regulated guanine nucleotide exchange factor I; EPAC; EPAC 1; EPAC1; exchange factor directly activated by cAMP 1; exchange protein directly activated by cAMP 1; HSU79275; Rap guanine nucleotide exchange factor (GEF) 3; rap guanine nucleotide exchange factor 3; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP
- HostRabbit
- IsotypeIgG
- Protein IDO95398
- Protein NameRap guanine nucleotide exchange factor 3
- Scientific DescriptionBoster Bio Anti-Epac1/RAPGEF3 Antibody Picoband® catalog # A02483-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Monkey, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203