Flow Cytometry: HCT-116 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66335 anti-EpCAM antibody [SQab1883] (red) at 1:800 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
![Immunohistochemistry: Formalin-fixed and paraffin-embedded Human uterus tissue stained with ARG66335 anti-EpCAM antibody [SQab1883] at 1:3,200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.](https://www.arigobio.com/files/product/16420/ARG66335_IHC-P_HuUterus.jpg "Immunohistochemistry: Formalin-fixed and paraffin-embedded Human uterus tissue stained with ARG66335 anti-EpCAM antibody [SQab1883] at 1:3,200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.")
![Immunoprecipitation: 0.4 mg of HT-29 whole cell lysate immunoprecipitated (1:50) and stained with ARG66335 anti-EpCAM antibody [SQab1883]. 1) ARG66335 IP in HT-29 whole cell lysate, 2) PBS instead of ARG66335 in HT-29 whole cell lysate, and 3) HT-29 whole cell lysate, 10 microg (input).](https://www.arigobio.com/files/product/16420/ARG66335_IP_HT-29.jpg "Immunoprecipitation: 0.4 mg of HT-29 whole cell lysate immunoprecipitated (1:50) and stained with ARG66335 anti-EpCAM antibody [SQab1883]. 1) ARG66335 IP in HT-29 whole cell lysate, 2) PBS instead of ARG66335 in HT-29 whole cell lysate, and 3) HT-29 whole cell lysate, 10 microg (input).")
![Western blot: 10 microg of HT-29 and T47D cell lysates stained with ARG66335 anti-EpCAM antibody [SQab1883] at 1:2000 dilution.](https://www.arigobio.com/files/product/16420/ARG66335_WB_1.jpg "Western blot: 10 microg of HT-29 and T47D cell lysates stained with ARG66335 anti-EpCAM antibody [SQab1883] at 1:2000 dilution.")
Flow Cytometry: HCT-116 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66335 anti-EpCAM antibody [SQab1883] (red) at 1:800 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
anti-EpCAM antibody [SQab1883]
ARG66335
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetEPCAM
Overview
- SupplierArigo Biolaboratories
- Product Nameanti-EpCAM antibody [SQab1883]
- Delivery Days Customer23
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDSQab1883
- ConjugateUnconjugated
- Gene ID4072
- Target nameEPCAM
- Target descriptionepithelial cell adhesion molecule
- Target synonymsadenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; DIAR5; EGP-2; EGP314; EGP40; epithelial cell adhesion molecule; epithelial glycoprotein 314; ESA; HNPCC8; human epithelial glycoprotein-2; KS1/4; KSA; M4S1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; trophoblast cell surface antigen 1; tumor-associated calcium signal transducer 1
- HostRabbit
- IsotypeIgG
- Scientific DescriptionEpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203