Anti-EpCAM [HEA125]

Absolute Antibody

Anti-EpCAM [HEA125]

9

This antibody is specific for the extracytoplasmic part of human EpCAM, a stable and widely-distributed marker expressed on the surface of many adenocarcinomas. It reacts with carcinomas derived from the colorectum, stomach, pancreas, liver, lung, mammary

This antibody has applications in the detection of normal and neoplastic epithelial cells, and distinguishing carcinomas from non-epithelial neoplasms (Momburg et al, 1987). This antibody has been used in radioimmunoassays on live cells and immunoenzymatic staining assays on glutaraldehyde-fixed cells, as well as in immunoperoxidase staining of frozen and formalin-fixed paraffin tissue sections (Moldenhauer et al, 1987; Momburg et al, 1987). These revealed it to react with most normal human epithelial cells, but not non-epithelial tissue, and to intensely react with human carcinomas and metastatic lesions, but not lymphoma, melanoma, sarcoma or neuroblastoma cell lines (Moldenhauer et al, 1987; Momburg et al, 1987). Squamous cell carcinomas stain less strongly than adenocarcinomas, and keratinizing areas of tumor masses remain negative (Momburg et al, 1987). This antibody has also been used in immunoprecipitation analysis to characterise human EpCAM (Moldenhauer et al, 1987), and in immunohistochemical analysis of EpCAM expression in formalin-fixed, paraffin-embedded liver tissue sections (Igarashi et al, 2013; Wang et al, 2018) and frozen liver tissue sections (Crosby et al, 1998). Additionally, it has been used in the immunocytochemical detection of EpCAM expression in mononuclear cell preparations obtained from human peripheral blood or lamina propria (Qiao et al, 1996).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, RadioImmunoAssay

Research Use Only

Monoclonal

HEA125

EPCAM

epithelial cell adhesion molecule

adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; DIAR5; EGP-2; EGP314; EGP40; epithelial cell adhesion molecule; epithelial glycoprotein 314; ESA; HNPCC8; human epithelial glycoprotein-2; KS1/4; KSA; M4S1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; trophoblast cell surface antigen 1; tumor-associated calcium signal transducer 1

Human

IgG1

Epithelial cell adhesion molecule

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203