Anti-EpCAM [KS1/4]

Absolute Antibody

Anti-EpCAM [KS1/4]

9

This antibody binds to the EpCAM receptor which is expressed on surface of adenocarcinomas and almost all epithelial cell membranes except mesodermal and neural cell membranes.

This antibody has been shown to be an effective agent for the invitro detection of disease and invivo diagnosis, prognosis and treatment of adenocarcinoma. Initial specificity of the antibody was determined by ELISA. Immunoperoxidase staining and immunofluorescence staining techniques were used to identify the reactivity of the antibody with various tissues (PMID: 6362852). The monoclonal antibody-vinca alkaloid conjugate, KS1/4-DAVLB (LY256787) were administered to nude mice bearing P3/UCLA human lung adenocarcinoma tumors. It was shown that KS1/4 monoclonal antibody targets DAVLB to the P3/UCLA human lung adenocarcinoma in vivo in the human xenograft model and that an increased therapeutic index may be achieved with LY256787 over conventional free drug therapy. (PMID: 3494841). KS1/4-DAVLB conjugates were also used for site directed therapy of epithelial malignancies (PMID: 2979063). Flow cytometric methods were used to evaluate of the cell surface binding properties of monoclonal antibody (KS1/4)-drug/toxin conjugates (PMID: 3492560). Further characterization of the antibody via immunoperoxidase techniques, flow cytometric analyses and solid phase enzyme-linked immunoassays suggested that this antibody represents an epithelial malignancy marker (PMID: 3049306). Phase I clinical trials revealed that 10 out of eleven patients had human anti-mouse response (Elias et al., 1990). Humanized version of this antibody is available on request.

Flow Cytometry, ELISA

Research Use Only

Monoclonal

KS1/4

EPCAM

epithelial cell adhesion molecule

adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; DIAR5; EGP-2; EGP314; EGP40; epithelial cell adhesion molecule; epithelial glycoprotein 314; ESA; HNPCC8; human epithelial glycoprotein-2; KS1/4; KSA; M4S1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; trophoblast cell surface antigen 1; tumor-associated calcium signal transducer 1

Mouse

IgG2b

Epithelial cell adhesion molecule

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203