Figure 1. Western blot analysis of Eph receptor A5 using anti-Eph receptor A5 antibody (PB9583). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Mouse Brain Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A5 antigen affinity purified polyclonal antibody (Catalog # PB9583) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A5 at approximately 114 kDa. The expected band size for Eph receptor A5 is at 114 kDa.
Figure 1. Western blot analysis of Eph receptor A5 using anti-Eph receptor A5 antibody (PB9583). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Mouse Brain Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A5 antigen affinity purified polyclonal antibody (Catalog # PB9583) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A5 at approximately 114 kDa. The expected band size for Eph receptor A5 is at 114 kDa.
Anti-Eph receptor A5/EPHA5 Antibody Picoband(r)
PB9583
ApplicationsWestern Blot
Product group Antibodies
ReactivityChicken, Human, Mouse, Rat
TargetEPHA5
Overview
- SupplierBoster Bio
- Product NameAnti-Eph Receptor A5 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2044
- Target nameEPHA5
- Target descriptionEPH receptor A5
- Target synonymsbrain-specific kinase; CEK7; EHK1; EHK-1; EK7; EPH homology kinase 1; EPH-like kinase 7; ephrin type-A receptor 5; epididymis secretory sperm binding protein; HEK7; receptor protein-tyrosine kinase HEK7; TYRO4; tyrosine-protein kinase receptor EHK-1
- HostRabbit
- IsotypeIgG
- Protein IDP54756
- Protein NameEphrin type-A receptor 5
- Scientific DescriptionBoster Bio Anti-Eph receptor A5/EPHA5 Antibody Picoband® catalog # PB9583. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityChicken, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203