Anti-EpHA3 [IIIA4]

Absolute Antibody

Anti-EpHA3 [IIIA4]

9

The antibody binds to the native EphA3 globular ephrin-binding domain. The antibody targets a site closely adjacent to the heterotetramerization site on the N-terminal of EPHA3s extracellular domain adjacent to the ligand-binding site and has a high affin

This antibody was used for detection of hek expressed on LK63 cells by flow cytometry. Hek antigen was immunoprecipitated using the antibody from 125I-labeled LK63 cell lysates (Boyd et al., 1992; PMID: 1737782). The specificity of the chimeric version of the antibody was confirmed by ELISA analysis (US8664365). In a LK63 xenograft model, administration of the antibody inhibited the tumor growth, decreasing the spreading from the bone marrow to the spleen and other organs and increases the latency of the disease. Similar effects were observed in LK63 EPHA3 knock down xenografts. Further, antibody treatment of a xenograft model using EPHA3-transfected Reh, showed reduction in the bone marrow engraftment and increases the latency of the disease (Charmsaz et al., 2016; PMID: 27922598). The antibody bound to the native EphA3 globular ephrin-binding domain with subnanomolar affinity (KD ~ 0.5 nM) (Smith et al.; 2004; PMID: 14660665). The antibody specifically stained EphA3 in 95% of human tumor tissues from a broad range of cancer types (Vail et al. 2014; PMID: 25125683). The humanized version of the antibody (KB004), was tested in a multi-center Phase 1/2 trial, in patients with EphA3-positive hematologic neoplasms, including Chronic Myelogenous Leukemia, Acute Myeloid Leukemia, Acute lymphoblastic leukemia, Myelodysplastic Syndrome.

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, Other Application

Research Use Only

Monoclonal

IIIA4

EPHA3

EPH receptor A3

EK4; EPH-like kinase 4; eph-like tyrosine kinase 1; ephrin type-A receptor 3; ETK; ETK1; HEK; HEK4; human embryo kinase 1; testicular tissue protein Li 64; TYRO4; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1

Human

IgG1

Ephrin type-A receptor 3

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Human, Mouse

-20°C,2°C to 8°C

12352203