Western blot analysis of ERBB3 using anti-ERBB3 antibody (PA1880). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 antigen affinity purified polyclonal antibody (Catalog # PA1880) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERBB3 at approximately 180KD. The expected band size for ERBB3 is at 148KD.
Western blot analysis of ERBB3 using anti-ERBB3 antibody (PA1880). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 antigen affinity purified polyclonal antibody (Catalog # PA1880) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERBB3 at approximately 180KD. The expected band size for ERBB3 is at 148KD.
Anti-ErbB 3/ERBB3 Antibody Picoband(r)
PA1880
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human
TargetERBB3
Overview
- SupplierBoster Bio
- Product NameAnti-ERBB3/Her3 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for ERBB3 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2065
- Target nameERBB3
- Target descriptionerb-b2 receptor tyrosine kinase 3
- Target synonymsc-erbB3; c-erbB-3; ErbB-3; erbB3-S; FERLK; HER3; human epidermal growth factor receptor 3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3; proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3; VSCN1
- HostRabbit
- IsotypeIgG
- Protein IDP21860
- Protein NameReceptor tyrosine-protein kinase erbB-3
- Scientific DescriptionBoster Bio Anti-ErbB 3/ERBB3 Antibody catalog # PA1880. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Human
- Reactivity SupplierHuman, Bovine
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203