Anti-F glycoprotein [12B2]

Absolute Antibody

Anti-F glycoprotein [12B2]

7

This antibody is reported to broadly neutralize nipah and hendra virus by blocking membrane fusion. Biolayer interferometry was used to characterize binding of the 12B2 Fab fragments to prefusion NiV F or HeV F ectodomain trimers immobilized on the surface of biosensors. The equilibrium dissociation constants (Kd) of 27.7 and 5.35 nM for attachment of Fab fragments of 12B2 to NiV F or HeV F, respectively. To further characterize this antibody, a kinetic assay was performed on HeV F- or NiV F- or NiV F S69A using the mouse version of this antibody. A cryo electron microscopy was performed on samples of the Fab fragment of this antibody bound to the F glycoprotein of the nipah virus (Dang et al, 2021; pmid:33927387). To analyze the ability of this antibody to prevent and cure disease, a fusion inhibition assay was performed on CHO745 cells transfected with HNV F and G glycoproteins using the mouse IgG version of this antibody. Further a plaque reduction assay was performed NiV-M, NiV-B or HeV using both the human and mouse IgG version of this antibody (Dang et al., 2021; PMID: 33927387).

Functional Assay, Neutralisation/Blocking

neutralize; functional assay

Research Use Only

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203