Anti-F protein [2E3]

Absolute Antibody

Anti-F protein [2E3]

9

The original antibody is specific for prefusion F protein. The antibody binds the protein through a conformational epitope spanning across three distinct sections of the RSV F protein: residues 419-434, 443-448, and 459-467. F-protein plays a pivotal role

The specificity of the Fab and IgG1 format of the antibody was confirmed by ELISA analysis (EC50 value for IgG1 at 9.6 ng/mL). Surface plasmon resonance based studies demonstrated that the antibody bound exclusively to the prefusion conformation of the RSV F protein with an affinity Kd = 13 nM for monovalent Fab. The bivalent Fab exhibited neutralizing activities against both RSV A (Long) and B (Washington) strains with IC50 at 53.4 ng/mL and 515 ng/mL, respectively. The human IgG1 format showed an improved neutralizing activity against the RSV A strain (IC50 at 11.4 ng/mL) and comparable against the RSV B (Washington) strain (IC50 at 520 ng/mL).

ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

2E3

Human

Fusion glycoprotein F0

Virus

-20°C,2°C to 8°C

12352203