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Western blot analysis of FABP1 expression in human fetal liver lysate (M02477). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP1 monoclonal antibody (Catalog # M02477) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP1
Western blot analysis of FABP1 expression in human fetal liver lysate (M02477). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP1 monoclonal antibody (Catalog # M02477) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP1
Western blot analysis of FABP1 expression in human fetal liver lysate (M02477). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP1 monoclonal antibody (Catalog # M02477) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP1

Anti-FABP1/L Fabp Rabbit Monoclonal Antibody

Research Use Only
M02477
Boster Bio
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetFABP1
Price on request
100 ul
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-FABP1/L Fabp Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AACB-6
  • Formulation
    Liquid
  • Gene ID2168
  • Target name
    FABP1
  • Target description
    fatty acid binding protein 1
  • Target synonyms
    FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; L-FABP; liver-type fatty acid-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07148
  • Protein Name
    Fatty acid-binding protein, liver
  • Scientific Description
    Boster Bio Anti-FABP1/L Fabp Rabbit Monoclonal Antibody catalog # M02477. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203