Western blot analysis of FABP1 expression in human fetal liver lysate (M02477). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP1 monoclonal antibody (Catalog # M02477) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP1
Anti-FABP1/L Fabp Rabbit Monoclonal Antibody
M02477
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetFABP1
Overview
- SupplierBoster Bio
- Product NameAnti-FABP1/L Fabp Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAACB-6
- FormulationLiquid
- Gene ID2168
- Target nameFABP1
- Target descriptionfatty acid binding protein 1
- Target synonymsFABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; L-FABP; liver-type fatty acid-binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP07148
- Protein NameFatty acid-binding protein, liver
- Scientific DescriptionBoster Bio Anti-FABP1/L Fabp Rabbit Monoclonal Antibody catalog # M02477. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse.
- ReactivityHuman, Mouse
- Storage Instruction-20°C
- UNSPSC12352203