Western blot analysis of CFH using anti-CFH antibody (M00562) in human plasma lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFH antigen affinity purified polyclonal antibody (Catalog # M00562) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Western blot analysis of CFH using anti-CFH antibody (M00562) in human plasma lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFH antigen affinity purified polyclonal antibody (Catalog # M00562) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Anti-Factor H CFH Monoclonal Antibody
M00562
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCFH
Overview
- SupplierBoster Bio
- Product NameAnti-Factor H Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDACCI-3
- FormulationLiquid
- Gene ID3075
- Target nameCFH
- Target descriptioncomplement factor H
- Target synonymsadrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1H; beta-1-H-globulin; CFHL3; complement factor H; factor H; factor H-like 1; FH; FHL1; H factor 1 (complement); H factor 2 (complement); HF; HF1; HF2; HUS
- HostRabbit
- IsotypeIgG
- Protein IDP08603
- Protein NameComplement factor H
- Scientific DescriptionBoster Bio Anti-Factor H CFH Monoclonal Antibody catalog # M00562. Tested in WB, ICC/IF applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203