Anti-Factor VIII [EL-14]

Absolute Antibody

Anti-Factor VIII [EL-14]

9

This antibody binds the C2 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects

The binding characterization of this antibody towards radiolabeled FVIII light chain and the C2 domain of factor VIII was done using immunoprecipitation. The inhibitory activity of this antibody against factor VIII was determined using a one-stage clotting assay. This antibody was capable of completely eliminating FVIII inhibition by CLB-CAg 117, a C2 domain directed anti-factor VIII antibody (PMID: 10627462). The scFv version of this antibody bound factor VIII light chain with a dissociation constant of Kd = 1.1 nM (PMID: 11929772).

Functional Assay, ImmunoPrecipitation, Neutralisation/Blocking

Research Use Only

Monoclonal

EL-14

F8

coagulation factor VIII

AHF; antihemophilic factor; coagulation factor VIII; coagulation factor VIII A1 domain; coagulation factor VIII C2 domain; coagulation factor VIII, procoagulant component; coagulation factor VIIIc; DXS1253E; F8B; F8C; factor VIII F8B; FVIII; HEMA

Coagulation factor VIII

This is a reformatted human scFv antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203