Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (PB9587). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SKOV3 whole cell lysates, Lane 3: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # PB9587) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 18, 20 kDa. The expected band size for FGF2 is at 18, 22, 30 kDa.
Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (PB9587). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SKOV3 whole cell lysates, Lane 3: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # PB9587) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 18, 20 kDa. The expected band size for FGF2 is at 18, 22, 30 kDa.
Anti-FGF2 Antibody Picoband(r)
PB9587-HRP
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Human, Rat
TargetFGF2
Overview
- SupplierBoster Bio
- Product NameAnti-FGF2 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID2247
- Target nameFGF2
- Target descriptionfibroblast growth factor 2
- Target synonymsbasic fibroblast growth factor bFGF; BFGF; FGF-2; FGFB; fibroblast growth factor 2; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; prostatropin
- HostRabbit
- IsotypeIgG
- Protein IDP09038
- Protein NameFibroblast growth factor 2
- Scientific DescriptionBoster Bio Anti-FGF2 Antibody Picoband® catalog # PB9587. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203