Western blot analysis of FGF21 using anti-FGF21 antibody (PA1673). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate, Lane 2: A431 Whole Cell Lysate, Lane 3: HEPA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PA1673) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 21KD. The expected band size for FGF21 is at 21KD.
Western blot analysis of FGF21 using anti-FGF21 antibody (PA1673). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate, Lane 2: A431 Whole Cell Lysate, Lane 3: HEPA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PA1673) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 21KD. The expected band size for FGF21 is at 21KD.
Anti-Fibroblast growth factor 21 FGF21 Antibody Picoband(r)
PA1673
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetFGF21
Overview
- SupplierBoster Bio
- Product NameAnti-FGF21 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID26291
- Target nameFGF21
- Target descriptionfibroblast growth factor 21
- Target synonymsfibroblast growth factor 21
- HostRabbit
- IsotypeIgG
- Protein IDQ9NSA1
- Protein NameFibroblast growth factor 21
- Scientific DescriptionBoster Bio Anti-Fibroblast growth factor 21 FGF21 Antibody catalog # PA1673. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Bovine
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203