Figure 1. Western blot analysis of FGF 23 using anti-FGF 23 antibody (PB9868). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF 23 antigen affinity purified polyclonal antibody (Catalog # PB9868) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF 23 at approximately 34 kDa. The expected band size for FGF 23 is at 28 kDa.
Figure 1. Western blot analysis of FGF 23 using anti-FGF 23 antibody (PB9868). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF 23 antigen affinity purified polyclonal antibody (Catalog # PB9868) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF 23 at approximately 34 kDa. The expected band size for FGF 23 is at 28 kDa.
Anti-FGF23 Antibody Picoband(r)
PB9868-DYLIGHT488
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetFgf23
Overview
- SupplierBoster Bio
- Product NameAnti-FGF23 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 488
- Gene ID64654
- Target nameFgf23
- Target descriptionfibroblast growth factor 23
- Target synonymsFGF-23; Fgf8b; fibroblast growth factor 23; fibroblast growth factor 8b
- HostRabbit
- IsotypeIgG
- Protein IDQ9EPC2
- Protein NameFibroblast growth factor 23
- Scientific DescriptionBoster Bio Anti-FGF23 Antibody Picoband® catalog # PB9868. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203