Anti-FGRIa [M2F12]

Absolute Antibody

Anti-FGRIa [M2F12]

7

To characterize this antibody a western blot was preformed on sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. Furthermore immunoprecipitation was preformed on the lysed sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. And finally a radioimmunityassay was preformed with the mouse version of this antibody on I125-labeled complexes (Xu et al, 1992; pmid:1325449). During research on the localization and behaviour of the FGF-1 protein the murine version of this antibody was used to immunoprecipitate FGFR and a 40-kDa complex it is part of (Feng et al, 1995; pmid:9244177). To research the localization of FGF-1 the murine version of this antibody was used to immunoprecipitate FGFR-1 in NIH3T3 cells and L6 cells (Prudovsky et al, 1994; pmid:7527394). While researching the aberrant branch formation of granule cell axons (mossy fiber sprouting) in the dentate gyrus of many patients with temporal lobe epilepsy and in animal models of epilepsy. The murine version of this antibody was used for immunocytochemistry and immunohistochemistry on cultured granule neurons from P3 sprague dawley rats (Shimada et al, 2016; pmid:27098696). To study FGF expression in RCC tissue immunohistochemistry was preformed on prospectively collected surgical samples of the tumor tissue using the murine version of this antibody (Volkova et al, 2021; pmid:33816695). To investigate the distribution and immunolocalization of fibroblast growth factors. Oviduct tissue from wistar rats was immunohistochemically analyzed using the murine version of this antibody (Alan and Liman, 2020; pmid:33772852). FGFR1 immunoreactivity was analyzed in frozen sections of normal human brain white matter and glioblastomas by using the murine version of this antibody (Morrison et al, 1994; pmid:8168112).

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, RadioImmunoAssay

Research Use Only

This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203