
Figure 1. Western blot analysis of FHIT using anti-FHIT antibody (PB9181). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHIT antigen affinity purified polyclonal antibody (Catalog # PB9181) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHIT at approximately 17 kDa. The expected band size for FHIT is at 17 kDa.
Anti-FHIT Antibody Picoband(r)

PB9181-10UG
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetFHIT
Overview
- SupplierBoster Bio
- Product NameAnti-FHIT Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for FHIT is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID2272
- Target nameFHIT
- Target descriptionfragile histidine triad diadenosine triphosphatase
- Target synonymsAP3A hydrolase; AP3Aase; bis(5'-adenosyl)-triphosphatase; diadenosine 5',5'''-P1,P3-triphosphate hydrolase; dinucleosidetriphosphatase; FRA3B
- HostRabbit
- IsotypeIgG
- Protein IDP49789
- Protein NameBis(5'-adenosyl)-triphosphatase
- Scientific DescriptionBoster Bio Anti-FHIT Antibody Picoband® catalog # PB9181. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203