Immunofluorescence: HeLa cells stained with ARG52280 anti-Fibrillarin antibody [38F3] (green) at 1:100 dilution, and costained with ARG52468 anti-Vimentin antibody (red) at 1:1000 dilution. DAPI (blue) for nuclear staining.
![Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG52280 anti-Fibrillarin antibody [38F3] at 1:500 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.](https://www.arigobio.com/files/product/4446/ARG52280_IF_Sheet_HeLa_170519_22M-5.jpg "Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG52280 anti-Fibrillarin antibody [38F3] at 1:500 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.")
![Western blot: HEK293 cytosol, HEK293 nuclear, NIH/3T3 cytosol and NIH/3T3 nuclear fractions stained with ARG52280 anti-Fibrillarin antibody [38F3] (green) at 1:500 dilution.](https://www.arigobio.com/files/product/4446/ARG52280_WB_1_191002.jpg "Western blot: HEK293 cytosol, HEK293 nuclear, NIH/3T3 cytosol and NIH/3T3 nuclear fractions stained with ARG52280 anti-Fibrillarin antibody [38F3] (green) at 1:500 dilution.")
![Western blot: 30 microg of HeLa cell lysate stained with ARG52280 anti-Fibrillarin antibody [38F3] at 1:1000 dilution.](https://www.arigobio.com/files/product/4446/ARG52280_WB_Sheet1_Rev1_160425.jpg "Western blot: 30 microg of HeLa cell lysate stained with ARG52280 anti-Fibrillarin antibody [38F3] at 1:1000 dilution.")
Immunofluorescence: HeLa cells stained with ARG52280 anti-Fibrillarin antibody [38F3] (green) at 1:100 dilution, and costained with ARG52468 anti-Vimentin antibody (red) at 1:1000 dilution. DAPI (blue) for nuclear staining.
anti-Fibrillarin antibody [38F3]
ARG52280
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityYeast
TargetNOP1
Overview
- SupplierArigo Biolaboratories
- Product Nameanti-Fibrillarin antibody [38F3]
- Delivery Days Customer23
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID38F3
- ConjugateUnconjugated
- Gene ID851548
- Target nameNOP1
- Target descriptionrRNA methyltransferase NOP1
- Target synonymsLOT3; rRNA methyltransferase NOP1; YDL014W
- HostMouse
- IsotypeIgG1
- Scientific DescriptionNop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli . The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin . Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable . This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
- ReactivityYeast
- Storage Instruction-20°C
- UNSPSC12352203