anti-Fibrillarin antibody [38F3]

Arigo Biolaboratories

anti-Fibrillarin antibody [38F3]

23

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen

Research Use Only

Monoclonal

38F3

Unconjugated

NOP1

rRNA methyltransferase NOP1

LOT3; rRNA methyltransferase NOP1; YDL014W

Mouse

IgG1

Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli . The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin . Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable . This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Yeast

-20°C

12352203