Anti-FimA [123-123-10]

Absolute Antibody

Anti-FimA [123-123-10]

7

The antibody reacts with conformational epitopes displayed by partially dissociated oligomers of FimA, but not with the 43 kDa FimA monomer. FimA is a structural protein of P. gingivalis fimbriae and is known to be involved in bacterial adherence to the h

The antibody reacted with conformational epitopes expressed by partially dissociated oligomers, but not with monomer as elucidated by Western blot analysis. Ascites fluid containing the antibody showed the inhibitory activity against P. gingivalis to saliva-coated hydroxyapatite beads, an in vitro model for the pellicle-coated tooth surface. For example, 400-fold diluted antibody inhibited the binding of the bacteria onto bead surfaces of 29.5% (Koh et al., 2009; PMID: 20157608). The specificity of this antibody (IgG2b) was confirmed by ELISA analysis. The original antibody detected FimA by western blot. The antibody significantly inhibited the binding of P. gingivalis to the surface of saliva-coated hydroxyapatite (SHA) beads by 49.79 +/- 5.0%, when 50 ng/mL antibody was used (P < 0.01). The antibody could be used as a basis for passive immunization against P. gingivalis-mediated periodontitis (Koh et al., 2011; PMID: 21223367).

Western Blot, ELISA, Neutralisation/Blocking

ELISA; WB; inhibition

Research Use Only

Monoclonal

123-123-10

Rabbit

IgG

Major fimbrium subunit FimA type-1

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Bacteria

Porphyromonas gingivalis

The original antibody was generated by immunizing BALB/c mice with FimA protein.

-20°C,2°C to 8°C

12352203