Anti-Flavivirus group antigen [2A10G6]

Absolute Antibody

Anti-Flavivirus group antigen [2A10G6]

9

The antibody recognises the E protein of Flavivirus. The antibody reacted with Dengue 1-4 virus. The antibody binds to an epitope within the highly conserved flavivirus fusion loop peptide, the 98DRXW101 motif.

The specificity of the antibody for the E protein was confirmed by ELISA analysis. The antibody recognized dengue virus prM-E antigen by indirect immunofluorescence. Hamster kidney cell line BHK-21 was used to verify the inhibitory effect of the antibody on multiple flavivirus infections. The results of cell neutralization experiments showed that antibody can effectively block a variety of flavivirus infections and the binding of BHK21 cells. In vivo protection experiments showed that antibodies against flavivirus envelope E protein can effectively protect suckling mice against dengue virus attack, and the survival rate and survival time are significantly improved (CN101891806B). The crystal structure of the Fab fragment of the antibody in complex with zika virus envelope protein was determined. The antibody bound to the ZIKV-E protein with a high binding affinity (Kd= 2.7 nM) and neutralized the ZIKV infection in vitro. Further, the antibody completely protects mice against the circulating ZIKV strain in vivo (Dai et al., 2016; PMID: 27158114).

ImmunoFluorescence, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

2A10G6

POLY

polyprotein

polyprotein; YFVgp1

Human

IgG1

Genome polyprotein

Virus

-20°C,2°C to 8°C

12352203