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Figure 1. Western blot analysis of FHL using anti-FHL antibody (A01258-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL antigen affinity purified polyclonal antibody (Catalog # A01258-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL at approximately 32KD. The expected band size for FHL is at 36KD.
Product group Antibodies
Boster Bio
Anti-FHL Fhl1 Antibody Picoband(r)A01258-2-PE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse, Rat
TargetFhl1
  • SizePrice
Figure 1. Western blot analysis of FHL using anti-FHL antibody (A01258-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL antigen affinity purified polyclonal antibody (Catalog # A01258-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL at approximately 32KD. The expected band size for FHL is at 36KD.
Product group Antibodies
Boster Bio
Anti-FHL Fhl1 Antibody Picoband(r)A01258-2
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse, Rat
TargetFhl1
  • SizePrice
Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-FHL1 Antibody Picoband(r)A01258-3-10UG
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-FHL1 Antibody Picoband(r)A01258-3-APC
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-FHL1 Antibody Picoband(r)A01258-3-BIOTIN
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-FHL1 Antibody Picoband(r)A01258-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-FHL1 Antibody Picoband(r)A01258-3-CY3
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-FHL1 Antibody Picoband(r)A01258-3-DYLIGHT488
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-FHL1 Antibody Picoband(r)A01258-3-DYLIGHT550
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-FHL1 Antibody Picoband(r)A01258-3-DYLIGHT594
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-FHL1 Antibody Picoband(r)A01258-3-FITC
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Figure 1. Western blot analysis of FHL1 using anti-FHL1 antibody (A01258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL1 antigen affinity purified polyclonal antibody (Catalog # A01258-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL1 at approximately 36 kDa. The expected band size for FHL1 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-FHL1 Antibody Picoband(r)A01258-3-HRP
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
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