Western blot analysis of FLT3LG using anti-FLT3LG antibody (PA1914). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLT3LG antigen affinity purified polyclonal antibody (Catalog # PA1914) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLT3LG at approximately 30KD. The expected band size for FLT3LG is at 26KD.
Western blot analysis of FLT3LG using anti-FLT3LG antibody (PA1914). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLT3LG antigen affinity purified polyclonal antibody (Catalog # PA1914) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLT3LG at approximately 30KD. The expected band size for FLT3LG is at 26KD.
Anti-Flt3 ligand/FLT3LG Antibody Picoband(r)
PA1914
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetFLT3LG
Overview
- SupplierBoster Bio
- Product NameAnti-Flt-3ligand Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for Flt-3ligand is approximately 5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2323
- Target nameFLT3LG
- Target descriptionfms related receptor tyrosine kinase 3 ligand
- Target synonymsFL; FLG3L; flt3 ligand; FLT3L; fms related tyrosine kinase 3 ligand; fms-related tyrosine kinase 3 ligand
- HostRabbit
- IsotypeIgG
- Protein IDP49771
- Protein NameFms-related tyrosine kinase 3 ligand
- Scientific DescriptionBoster Bio Anti-Flt3 ligand/FLT3LG Antibody catalog # PA1914. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Reactivity SupplierHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203