Figure 1. Western blot analysis of FMO5 using anti-FMO5 antibody (PA2108). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO5 antigen affinity purified polyclonal antibody (Catalog # PA2108) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO5 at approximately 60 kDa. The expected band size for FMO5 is at 60 kDa.
Figure 1. Western blot analysis of FMO5 using anti-FMO5 antibody (PA2108). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO5 antigen affinity purified polyclonal antibody (Catalog # PA2108) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO5 at approximately 60 kDa. The expected band size for FMO5 is at 60 kDa.
Anti-FMO5 Antibody Picoband(r)
PA2108
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetFMO5
Overview
- SupplierBoster Bio
- Product NameAnti-FMO5 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2330
- Target nameFMO5
- Target descriptionflavin containing dimethylaniline monoxygenase 5
- Target synonymsbaeyer-Villiger monooxygenase 1; dimethylaniline monooxygenase [N-oxide-forming] 5; dimethylaniline oxidase 5; flavin containing monooxygenase 5; flavin-containing monooxygenase 5; FMO 5; hBVMO1; hepatic flavin-containing monooxygenase 5; NAPDH oxidase
- HostRabbit
- IsotypeIgG
- Protein IDP49326
- Protein NameDimethylaniline monooxygenase [N-oxide-forming] 5
- Scientific DescriptionBoster Bio Anti-FMO5 Antibody catalog # PA2108. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Reactivity SupplierHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203