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ProductsBack/Anti-Fos B/FOSB Antibody Picoband(r)
Figure 1. Western blot analysis of FOSB using anti-FOSB antibody (RP1086). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse RAW246.7 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOSB antigen affinity purified polyclonal antibody (Catalog # RP1086) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOSB at approximately 35KD. The expected band size for FOSB is at 35KD.
Figure 1. Western blot analysis of FOSB using anti-FOSB antibody (RP1086). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse RAW246.7 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOSB antigen affinity purified polyclonal antibody (Catalog # RP1086) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOSB at approximately 35KD. The expected band size for FOSB is at 35KD.
Figure 1. Western blot analysis of FOSB using anti-FOSB antibody (RP1086). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse RAW246.7 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOSB antigen affinity purified polyclonal antibody (Catalog # RP1086) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOSB at approximately 35KD. The expected band size for FOSB is at 35KD.

Anti-Fos B/FOSB Antibody Picoband(r)

Research Use Only
RP1086
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetFOSB
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Fos B Antibody
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Formulation
    Lyophilized
  • Gene ID2354
  • Target name
    FOSB
  • Target description
    FosB proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    activator protein 1; AP-1; FBJ murine osteosarcoma viral oncogene homolog B; FosB proto-oncogene, AP-1 trancription factor subunit; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; protein fosB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53539
  • Protein Name
    Protein fosB
  • Scientific Description
    Boster Bio Anti-Fos B/FOSB Antibody catalog # RP1086. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Hamster, Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203