Figure 1. Western blot analysis of FSH beta using anti-FSH beta antibody (PB9106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours Lane 1: recombinant human FSH beta protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSH beta antigen affinity purified polyclonal antibody (Catalog # PB9106) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSH beta at approximately 36 kDa. The expected band size for FSH beta is at 36 kDa.
Figure 1. Western blot analysis of FSH beta using anti-FSH beta antibody (PB9106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours Lane 1: recombinant human FSH beta protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSH beta antigen affinity purified polyclonal antibody (Catalog # PB9106) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSH beta at approximately 36 kDa. The expected band size for FSH beta is at 36 kDa.
Anti-FSH beta/FSHB Antibody Picoband(r)
PB9106
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetFSHB
Overview
- SupplierBoster Bio
- Product NameAnti-FSH Beta Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for FSH beta is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2488
- Target nameFSHB
- Target descriptionfollicle stimulating hormone subunit beta
- Target synonymsfollicle stimulating hormone beta subunit; follicle stimulating hormone, beta polypeptide; follitropin subunit beta; follitropin, beta chain; FSH-B; FSH-beta; HH24
- HostRabbit
- IsotypeIgG
- Protein IDP01225
- Protein NameFollitropin subunit beta
- Scientific DescriptionBoster Bio Anti-FSH beta/FSHB Antibody Picoband® catalog # PB9106. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse
- Reactivity SupplierHuman, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203