Western blot analysis of Galectin 1 using anti-Galectin 1 antibody (A00470). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse ovary tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: rat gaster tissue lysates, Lane 8: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 1 antigen affinity purified polyclonal antibody (Catalog # A00470) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15KD. The expected band size for Galectin 1 is at 15KD.
Western blot analysis of Galectin 1 using anti-Galectin 1 antibody (A00470). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse ovary tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: rat gaster tissue lysates, Lane 8: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 1 antigen affinity purified polyclonal antibody (Catalog # A00470) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15KD. The expected band size for Galectin 1 is at 15KD.
Anti-Galectin 1/Lgals1 Antibody Picoband(r)
A00470
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetLgals1
Overview
- SupplierBoster Bio
- Product NameAnti-Galectin 1 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID16852
- Target nameLgals1
- Target descriptionlectin, galactose binding, soluble 1
- Target synonyms14 kDa lectin; AA410090; beta-galactoside binding protein; beta-galactoside-binding lectin L-14-I; Ga; Gal; Gal-1; galaptin; Galbp; galect; galectin-1; L1; L14; L-14.5; lactose-binding lectin 1; Lect1; Lect14; lectin galactoside-binding soluble 1; S-Lac lectin 1
- HostRabbit
- IsotypeIgG
- Protein IDP16045
- Protein NameGalectin-1
- Scientific DescriptionBoster Bio Anti-Galectin 1/Lgals1 Antibody Picoband® catalog # A00470. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Trichinella spiralis co-infection exacerbates Plasmodium berghei malaria-induced hepatopathy. Mei X et al., 2020 Sep 3, Parasit VectorsRead more