Western blot analysis of GATA1 expression in K562 cell lysate (M00408). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA1 monoclonal antibody (Catalog # M00408) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA1
Anti-GATA1 Rabbit Monoclonal Antibody
M00408
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGATA1
Overview
- SupplierBoster Bio
- Product NameAnti-GATA1 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDABCB-7
- FormulationLiquid
- Gene ID2623
- Target nameGATA1
- Target descriptionGATA binding protein 1
- Target synonymsERYF1; erythroid transcription factor; erythroid transcription factor 1; GATA-1; GATA-binding factor 1; GF1; GF-1; globin transcription factor 1; NFE1; NF-E1; NF-E1 DNA-binding protein; nuclear factor, erythroid 1; transcription factor GATA1; XLANP; XLTDA; XLTT
- HostRabbit
- IsotypeIgG
- Protein IDP15976
- Protein NameErythroid transcription factor
- Scientific DescriptionBoster Bio Anti-GATA1 Rabbit Monoclonal Antibody catalog # M00408. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203