Figure 1. Western blot analysis of GATA3 using anti-GATA3 antibody (PB9199). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human GATA3 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 antigen affinity purified polyclonal antibody (Catalog # PB9199) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 39 kDa. The expected band size for GATA3 is at 39 kDa.
Figure 1. Western blot analysis of GATA3 using anti-GATA3 antibody (PB9199). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human GATA3 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 antigen affinity purified polyclonal antibody (Catalog # PB9199) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 39 kDa. The expected band size for GATA3 is at 39 kDa.
Anti-GATA3 Antibody Picoband(r)
PB9199-10UG
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetGATA3
Overview
- SupplierBoster Bio
- Product NameAnti-GATA3 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for GATA3 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID2625
- Target nameGATA3
- Target descriptionGATA binding protein 3
- Target synonymsGATA-binding factor 3; HDR; HDRS; trans-acting T-cell-specific transcription factor GATA-3
- HostRabbit
- IsotypeIgG
- Protein IDP23771
- Protein NameTrans-acting T-cell-specific transcription factor GATA-3
- Scientific DescriptionBoster Bio Anti-GATA3 Antibody Picoband® catalog # PB9199. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203