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Figure 1. Western blot analysis of GATM using anti-GATM antibody (A02754-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human AGS whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat HBZY-1 whole cell lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATM antigen affinity purified polyclonal antibody (Catalog # A02754-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATM at approximately 48 kDa. The expected band size for GATM is at 48 kDa.
Figure 1. Western blot analysis of GATM using anti-GATM antibody (A02754-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human AGS whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat HBZY-1 whole cell lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATM antigen affinity purified polyclonal antibody (Catalog # A02754-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATM at approximately 48 kDa. The expected band size for GATM is at 48 kDa.
Figure 1. Western blot analysis of GATM using anti-GATM antibody (A02754-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human AGS whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat HBZY-1 whole cell lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATM antigen affinity purified polyclonal antibody (Catalog # A02754-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATM at approximately 48 kDa. The expected band size for GATM is at 48 kDa.

Anti-GATM Antibody Picoband(r)

A02754-1-DYLIGHT594
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGATM
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-GATM Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Other Conjugate
  • Gene ID2628
  • Target name
    GATM
  • Target description
    glycine amidinotransferase
  • Target synonyms
    AGAT; AT; CCDS3; FRTS1; glycine amidinotransferase (L-arginine:glycine amidinotransferase); glycine amidinotransferase, mitochondrial; testicular secretory protein Li 19; transamidinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50440
  • Protein Name
    Glycine amidinotransferase, mitochondrial
  • Scientific Description
    Boster Bio Anti-GATM Antibody Picoband® catalog # A02754-1. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203