Anti-gelatinase B [REGA-3G12]

Absolute Antibody

Anti-gelatinase B [REGA-3G12]

9

This antibody is specific for human gelatinase B; it recognizes the catalytic site region between Trp116 and Lys214 of the enzymes globular aminoterminal part. A dimer scFv (with a His6 tag) variant of this antibody was found to cross-react with gelatinas

The specificity of this antibodys original format (mouse IgG1, kappa) was evaluated by ELISA; it specifically bound to gelatinase B with a binding affinity of 2.1 x 10-9 M. This antibody detected both native and H2NPhAcHg-treated gelatinase B in an immunoblot, both in non-reducing and reducing conditions. Furthermore, this antibody was found to inhibit gelatinase B activity in vitro (Paemen et al., 1995; PMID: 8575432). The binding of the scFv version of this antibody to gelatinase B was confirmed in an ELISA. The original antibody and its F(ab)2 and scFv derivatives inhibited gelatinase B in vitro (Zhou et al., 1997; PMID: 9323036). The following scFv derivatives of this antibody were evaluated for their inhibitory effect toward gelatinase B: REGA-3G12 scFv with (His6) monomer (10 microM), REGA-3G12 scFv with (His6) dimer (5 microM), and REGA-3G12 scFv without a tag (1.25, 2.5, and 5 microM). They achieved 6.5%, 65.9%, 8.8%, 32.4%, and 44.4% inhibitory activity, respectively. Moreover, the scFv His-tag dimer variant was found to cross-react with gelatinase A with a 75% inhibitory activity (same antibody concentration) (Hu et al., 2004; PMID: 15104254). The original format of the antibody was used to detect and characterize the binding of this antibody to gelatinase B via immunoprecipitation (IP) and Western blot (WB), and the catalytic domain in the aminoterminal segment of gelatinase B was identified as its target (Martens et al., 2007; PMID: 17137715).

ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

REGA-3G12

MMP9

matrix metallopeptidase 9

CLG4B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase-9; MMP-9; type V collagenase

Rabbit

IgG

Matrix metalloproteinase-9

Human

-20°C,2°C to 8°C

12352203