IHC analysis of GFAP using anti-GFAP antibody (PB9082). GFAP was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of GFAP using anti-GFAP antibody (PB9082). GFAP was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-GFAP Antibody Picoband(r)
PB9082
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGFAP
Overview
- SupplierBoster Bio
- Product NameAnti-GFAP Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for GFAP is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2670
- Target nameGFAP
- Target descriptionglial fibrillary acidic protein
- Target synonymsALXDRD; glial fibrillary acidic protein
- HostRabbit
- IsotypeIgG
- Protein IDP14136
- Protein NameGlial fibrillary acidic protein
- Scientific DescriptionBoster Bio Anti-GFAP Antibody Picoband® catalog # PB9082. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Pig. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
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- Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model. Zhang LY et al., 2021 Aug, Neural Regen ResRead more
- Tak1 in the astrocytes of mediobasal hypothalamus regulates anxiety-like behavior in mice. Zhao F et al., 2021 Mar, GliaRead more
- alpha7nAchR mediates transcutaneous auricular vagus nerve stimulation-induced neuroprotection in a rat model of ischemic stroke by enhancing axonal plasticity. Li J et al., 2020 Jun 21, Neurosci LettRead more
- Protective effects of ghrelin on brain mitochondria after cardiac arrest and resuscitation. Xu H et al., 2019 Aug, NeuropeptidesRead more
- The neuroprotective effects and probable mechanisms of Ligustilide and its degradative products on intracerebral hemorrhage in mice. Han L et al., 2018 Oct, Int ImmunopharmacolRead more