Anti-GFP [Minimizer]

Absolute Antibody

Anti-GFP [Minimizer]

9

The antibody is specific for green fluorescent protein. The antibody interacts with beta-strands 6 and 7 of GFP.

The antibody could reversibly reduce GFP fluorescence by a factor of 5 in vitro. A comparable fluorescence modulation was also observed after addition of the antibody to soluble cell extract derived from human embryonic kidney (HEK) 293T cells expressing eGFP. The crystal structure of the GFP-antibody complex was determined. The dissociation constant of the antibody was measured (Kd= 0.45 nM) (Kirchhofer et al., 2010; PMID: 20010839). Fluorescence modulation in living cells was also observed after the addition of the antibody to HeLa cells expressing GFP (Schmidthals et al., 2010; PMID: 20372881).

Other Application

Research Use Only

Monoclonal

Minimizer

Rabbit

IgG

Green fluorescent protein

Jellyfish

-20°C,2°C to 8°C

12352203