Anti-GFP Tag (Mouse Monoclonal)

Applied Biological Materials

Anti-GFP Tag (Mouse Monoclonal)

9

Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.

Western Blot, ELISA

Research Use Only

Monoclonal

LGB-1

Mouse

IgG1

Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

-20°C,2°C to 8°C

12352203