Anti-gH [YC15]

Absolute Antibody

Anti-gH [YC15]

9

YC15 was shown to bind ectopically-expressed gH on transfected cell lines, as well as neutralise infection by KSHV virions (unpublished observations). Kaposis sarcoma is a rare type of cancer, characterised by neoplasmic growth, often associated with AIDS

The YC15 variable domain sequences were cloned and redesigned into the scFv format (Cai & Berger, 2011), and further fused to PE38, a construct of Pseudomonas aeruginosa exotoxin A which is lacking the N-terminal cell adhesion domain. In this format, YC15 showed specific dose-dependent binding to surface-expressed gH using flow cytometry. As an immunotoxin with the fused P. aeruginosa PE38 construct, YC15 facilitated potent cytotoxic activity based on recognition of surface gH epitopes.

Flow Cytometry, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking

Research Use Only

Monoclonal

YC15

ORF22

ORF22

HHV8GK18_gp26; ORF22

Human

IgG1

Envelope glycoprotein H

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203