Anti-gHgL [CL59]

Absolute Antibody

Anti-gHgL [CL59]

9

The antibody binds an epitope in gH D-IV, proximal to the viral membrane and distant from known receptor binding sites. In particular, the antibody binds to the back side of gH D-III/D-IV close to gH D-III residues 494-503 (loop between 3alpha-8 and 3alph

The antibody blocks EBV-mediated membrane fusion with both B cells and epithelial cells. Flow cytometry cell counts showed that Fab fragment inhibits virus entry. Neutralization experiments with epithelial cells using an EBV-GFP reporter virus and purified Fab. Fluorescence images obtained at 24 h postinfection and flow cytometry cell counts showed that the Fab inhibits virus entry. The Fab exhibited ~50% inhibition of EBV infection at 150 nM, where no inhibition of cell-cell membrane fusion was observed. (PMID: 28939750) The cell-surface expression of gHgL was evaluated using the antibody by CELISA. (PMID: 27929061)

Flow Cytometry, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

CL59

BKRF2

envelope glycoprotein L

envelope glycoprotein L; HHV4_BKRF2

Human

IgG1

Envelope glycoprotein L

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Virus

-20°C,2°C to 8°C

12352203